DNA Sequence Assembly

Resource Type
Description
This tutorial describes the principles of DNA sequencing and sequence assembly, and shows how they apply to different sequencing techniques.
The speed of DNA sequencing has greatly increased, and the cost has substantially decreased, over the years. This Click & Learn reviews three milestone methods that advanced the development of DNA sequencing technology: Sanger sequencing, shotgun sequencing, and ultra-deep sequencing. Although not all of these methods are widely used today, the basic concepts of how DNA sequences are assembled still apply.
The accompanying worksheet guides students’ exploration.
Student Learning Targets
Describe Sanger sequencing, shotgun sequencing, and deep sequencing.
Estimated Time
Key Terms
bioinformatics, computation, consensus sequence, laboratory technique, next-generation sequencing, nucleotide, overlap sequence, primer, reference genome, whole genome sequencing
Terms of Use
Please see the Terms of Use for information on how this resource can be used.
Version History
NGSS (2013)
HS-LS1-1; SEP6
AP Biology (2019)
IST-1.L, IST-1.P, SYI-1.B, SYI-1.C; SP1
IB Biology (2016)
7.1, B.5
Common Core (2010)
ELA.RST.9-12.2; ELA.WHST.9-12.9
Vision and Change (2009)
CC2; DP1