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DNA Cloning with Plasmids

Topic

  • Biochemistry & Molecular Biology
  • DNA & RNA
  • Biotechnology
  • Microbiology
  • Bacteria

Resource Type

  • Videos
  • Animations

Level

High School — AP/IBCollege

Duration

00:01:13
Saved By
11 Users
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Description

This animation describes a genetic engineering technique called DNA cloning, which can be used to make bacteria express a foreign gene, typically from another species.

During DNA cloning, a new gene is inserted into a loop of bacterial DNA called a plasmid. As shown in the animation, the plasmid is first cut with a restriction enzyme so that the gene of interest, which is isolated from another organism, can be inserted into the loop. The loose ends of the DNA are then stitched together by an enzyme called DNA ligase. Bacteria that have incorporated the resulting plasmid will manufacture the protein encoded by the target gene.

Depending on students’ background, it may be helpful to pause the animation at various points to discuss different structures or steps in the method.

Key Terms

DNA ligase, EcoR1, gene insertion, genetic engineering, laboratory technique, protein, recombinant DNA, restriction enzyme, vector

Terms of Use

The resource is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license. No rights are granted to use HHMI’s or BioInteractive’s names or logos independent from this Resource or in any derivative works.

Accessibility Level (WCAG compliance)

Video files partially meet criteria.

Version History

Date Published 02.05.03

Materials

Large (MOV) 9 MB
Large (WMV) 9 MB
Small (MOV) 3 MB
Small (WMV) 3 MB
Transcript (PDF) 12 KB

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