Bacterial Identification Virtual Lab
This interactive, modular lab explores the techniques used to identify different types of bacteria based on their DNA sequences.
In this lab, students prepare and analyze a virtual bacterial DNA sample. In the process, they learn about several common molecular biology methods, including DNA extraction, PCR, gel electrophoresis, and DNA sequencing and analysis. These methods are applicable in a wide variety of settings, including scientific research and forensic labs.
The lab contains an interactive lab space and an informational notebook with detailed procedures. It also includes supplementary resources, such as a glossary of scientific terms, images of equipment and tools, and an encyclopedia of bacteria.
The accompanying worksheet provides structure and guidance as students perform the procedures in the lab.
Student Learning Targets
- Explain how bacteria can be identified using 16S rDNA sequences.
- Describe the steps for isolating, copying, and sequencing a DNA sample.
16S ribosomal RNA (16S rRNA), bioinformatics, BLAST search, DNA extraction, gel electrophoresis, laboratory technique, pathology, polymerase chain reaction (PCR), sequencing
The resource is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International license. No rights are granted to use HHMI’s or BioInteractive’s names or logos independent from this Resource or in any derivative works.
HS-LS1-1, HS-LS3-1, HS-LS4-1; SEP2, SEP3
1.A.3, 3.A.1; 1 SP1, SP4, SP7
5.3, 6.3, 11.1, B.4, B.5
ELA.RST.9–12.2, ELA.RST.9–12.4, ELA.RST.9–12.3, ELA.WHST.9–12.1
Math.A-REI.3; MP2, MP3
CC1, CC5; DP1, DP3, DP4, DP6